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以‘突尼斯软籽’石榴为试材,对雌蕊败育的原因及关键时期、PgAGL11序列特征及功能进行研究。石蜡切片观察结果表明,雌蕊败育的原因为胚珠内珠被原基形成后停止发育,胚珠败育的关键时期为花蕾纵径8.1~15.0 mm时;采用TA克隆得到PgAGL11全长CDS序列696 bp,系统进化分析发现其与拟南芥的AGL11序列相似性最高;实时荧光定量PCR结果显示,PgAGL11在雌蕊中表达量显著高于其他花器官,且在胚珠败育关键时期(花蕾纵径8.1~15.0 mm),可育花雌蕊中表达量极显著高于败育花雌蕊;构建PgAGL11过量表达载体,利用农杆菌介导法侵染野生型拟南芥,转基因拟南芥花表现为雄蕊变短,花瓣变小,花柱变长,柱头表面乳突状物质变长。本研究确定了石榴雌蕊败育的关键原因及时期,并初步证明了PgAGL11可能在石榴雌蕊败育过程中发挥重要作用。
Using ’Tunisian soft seeds’ pomegranate as test material, the reasons for the abortion of pistils and the key period of pistil function were studied. The observation of paraffin sections showed that the reason for the abortion of pistils was that the primordium of pgagl11 stopped forming after the formation of the primordium, and the key period of ovule abortion was 8.1 ~ 15.0 mm in length. The length of CDg sequence of PgAGL11 was 696 bp The results of real-time PCR showed that the expression level of PgAGL11 in pistils was significantly higher than that in other flower organs, and at the key stage of ovule abortion (the longitudinal length of flower buds was 8.1 ~ 15.0 mm). The expression level of pgAGL11 in pistils was significantly higher than that in aborted pistils. The PgAGL11 overexpression vector was constructed and Agrobacterium-mediated invasion of wild-type Arabidopsis thaliana was observed. , Petals smaller, styles longer, stigma surface mastoid material becomes longer. This study determined the key reasons and periods of pistil abortion of pomegranate and preliminarily proved that PgAGL11 may play an important role in the abortion of pomegranate pomegranate.