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作者应用多种不同的生物基质以观察它们对鼠乳房肿瘤细胞的无血清培养的影响,现将结果报道如下。材料和方法一、细胞林和CD_8F1杂交瘤细胞的原代培养。FUKU细胞是源自于BALB/cfC3H鼠乳房腺癌的表皮细胞细胞株。其培养体系为DMFM和Ham’S F12以1:1(?)
The authors used a variety of different biological matrices to observe their effect on serum-free culture of rat mammary tumor cells, and the results are reported below. Materials and Methods 1. Primary cultures of cell lines and CD_8F1 hybridoma cells. FUKU cells are epidermal cell lines derived from BALB/cfC3H murine mammary adenocarcinoma. The culture system is 1:1 (?) for DMFM and Ham’S F12.