利什曼原虫无鞭毛期主要寄生在人体内巨噬细胞和网状内皮系统的其它吞噬细胞内。通常,媒介体内的前鞭毛体可经体外培养获得,而对无鞭毛期原虫的体外培养研究自1979年以来曾有不少学者用kuffer′s细胞、皮肤粘膜细胞、骨髓细胞及人外周血液等方法,均只能在较短时间获得成功。国内对无鞭毛期的体外培养,迄今尚无报道。为此,我们对其培养方法进行了研究,并以获得的材料,对杜氏利什曼原虫无鞭毛期的相关抗原作了观察。 The Leishmania nonflagellate stage is mainly parasitic in human macrophages and other phagocytic cells of the reticuloendothelial system. In general, promastigotes in media can be obtained by in vitro culture, and in vitro culture of non-flagellated protozoa has been used by many scholars since 1979, including kuffer’s cells, mucocutaneous cells, bone marrow cells and human peripheral blood Method, can only be successful in a relatively short period of time. Domestic non-flagellum in vitro culture, so far no report. To this end, we conducted a study on their cultivation methods and observed the relevant antigens of Leishmania donovani in the absence of flagella with the obtained materials.