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小鼠睾丸组织经研磨制成细胞悬浮液,按常规法分别培养4、24、48、和72小时后制片,统计出生精细胞中终变期、中期细胞所占的百分数,并与未经培养组(对照组1)及活体内注射秋水仙素后取材制片组(对照组2相比较。结果表明:经4、24小时培养的分裂指数分别为0.44±0.15、1.16±0.52,比对照组1(0.16±0.05)有明显升高;培养4小时组与对照组2(0.42±0.16)相近,但培养24小时组则高于对照组2(P<0.05)。本实验证实:小鼠睾丸细胞在体外经4—24小时的培养,也可达到增加可供染色体分析细胞数的目的。
Mice testicular tissue was made into a cell suspension by grinding, according to the conventional method were cultured 4,24,48, and 72 hours after the production, statistics of spermatogenic cells in the terminal metaphase, the percentage of cells in the middle period, Compared with control group 2, the culture group (control group 1) and the colchicine group (control group 2) were inoculated in vivo.The results showed that the indices of division after 4 and 24 hours were 0.44 ± 0.15 and 1.16 ± 0.52 respectively, Group 1 (0.16 ± 0.05) was significantly higher than that of control group 2 (0.42 ± 0.16), but it was higher in 24-hour group than that in control group 2 (P <0.05) .This experiment confirmed that mice Testicular cells in vitro by 4-24 hours of culture, but also can increase the number of cells available for chromosome analysis purposes.