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当今,吸收现代多学科的长处研究中医,打破过去只从本学科范围内进行研究的状况,是时代的要求。按照中医理论,曾用驴和大鼠复制脾气虚证动物模型。本课题研究目的是以中药和饮食失节复制动物模型,再用健脾中药使其复健,观察驴心肌的超微结构变化。实验动物驴,用大黄、厚朴、积实和水灌胃,隔日一次,灌胃日饥饿,共6周。对照组灌水,正常饲养。经中医辨证确认为脾气虚证后,用加味四君子汤使其复健。自然恢复组灌水,正常饮食。分别取正常驴、脾气虚证驴、加味四君子汤复健驴以及自然恢复驴的左心室心肌,按常规制作透射电镜样品。脾气虚证驴的心肌纤维在电镜下观察,与正常的(图1)相比,超微结构主要改变是线粒体,图2显
Nowadays, it is the requirements of the times to absorb the strengths of modern multidisciplinary research into Chinese medicine and to break the previous situation of conducting research within the scope of this discipline. According to traditional Chinese medicine theory, the donkey and rat were used to copy the animal model of spleen deficiency syndrome. The purpose of this study is to reproduce the animal model of traditional Chinese medicine and diet disorders, and then re-use of Chinese herbal medicine to observe the ultrastructure of donkey myocardium changes. Experimental animals donkey, with rhubarb, Magnolia, plot and water gavage, every other day, gavage day hunger, a total of 6 weeks. Control group, normal feeding. Confirmed by the TCM differentiation of spleen qi deficiency syndrome, with the addition of Sijunzitang rehabilitation. Natural recovery group irrigation, normal diet. Were taken normal donkey, spleen qi donkey, add four Soup Donkey rehabilitation and donkey natural recovery of the left ventricular myocardium, according to conventional production of transmission electron microscopy samples. Myocardial fibers in the Spleen Deficiency Syndrome were observed under electron microscopy, and the major change in ultrastructure was mitochondria compared to normal (Figure 1), Figure 2