【摘 要】
：
目的探讨PTEN基因在动物体内对脑胶质瘤的生长作用。方法将大鼠C6胶质瘤细胞(对照组)和转染PTENcDNA的C6细胞(转染组)种植于SD大鼠右侧尾状核,荷载C6脑胶质瘤鼠用PTENcDNA原
【机 构】
：
天津市环湖医院神经外科,天津医科大学总医院神经外科,天津医科大学总医院神经外科,天津医科大学总医院神经外科,天津医科大学总医院神经外科,天津医科大学总医院神经外科,
论文部分内容阅读
目的探讨PTEN基因在动物体内对脑胶质瘤的生长作用。方法将大鼠C6胶质瘤细胞(对照组)和转染PTENcDNA的C6细胞(转染组)种植于SD大鼠右侧尾状核,荷载C6脑胶质瘤鼠用PTENcDNA原位治疗(治疗组),并以空载体治疗作为对照(空载组),每组10只,观察大鼠的一般情况、生存期、肿瘤体积变化以及肿瘤病理组织学与细胞生物学特征变化。结果对照组和空载组大鼠均于3周内死亡,而转染组5只大鼠和治疗组6只大鼠观察60d内无自然死亡,生存期较对照组明显延长(P<0.01)。结论PTEN基因在动物体内可以抑制脑胶质瘤的生长,可以成为恶性胶质瘤基因治疗的优选靶之一。
Objective To investigate the effect of PTEN gene on the growth of glioma in animals. Methods Rat C6 glioma cells (control group) and C6 cells transfected with PTEN cDNA (transfection group) were implanted in the right caudate nucleus of SD rats. C6 glioma mice were treated with PTEN cDNA in situ Group). The empty vector group was used as a control (no-load group), with 10 rats in each group. The general condition, survival time, tumor volume change and the change of tumor histopathology and cell biology were observed. Results The rats in the control group and the no-load group all died within 3 weeks, while 5 rats in the transfection group and 6 rats in the treatment group showed no natural death within 60 days. The survival time was significantly longer than that in the control group (P <0.01) . Conclusion PTEN gene can inhibit the growth of glioma in animal and it may be one of the preferred targets for gene therapy of malignant glioma.
其他文献
In order to apply Satellite Remote Sensing (RS) to mining areas, some key issues should be solved. Based on an introduction to relative studying background, rel
通过编辑实践经验总结,对技术类期刊正文转接的处理原则、适宜处理时期和具体实施技巧进行提炼,旨在提高编辑工作效率和期刊质量。
Through editing the summary of practic
In this paper, a new timing offset estimation method for Orthogonal Frequency Division Multiplexing (OFDM) systems are presented. Upon the receipt of just one t
A large-scale flume (40 cm×10 cm×10 cm) was used to determine soil anti-scourability of 4 different land uses of purple soil in hilly areas of Sichuan, China
Severe acute respiratory syndrome (SARS) is a serious disease with many puzzling features. We present a simple, dynamic model to assess the epidemic potential o
The effect of acute E. coli challenge on the anti-disease activity of scallops Argopecten irradians is examined. The treatments of scallop from which hemolymph
Landslide prediction is one of the most important aspects of prevention and control for geological hazards and the environmental protection. In order to study t
Objective To indicate the deficiency of the classical method for analyzing data on individual matching case-control study in consideration of the interaction be
A numerical methodology for investigating compressor instabilities in a multistage environment is presented. The method is based on a stage-by-stage dynamic com
Objective To investigate phosphorus limitation and its effect on the removal efficiency of organic matters in drinking water biological treatment. Methods Bacte