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目的了解敖汉旗境内鼠类,家畜家禽携带小肠结肠炎耶尔森菌(以下简称为小肠结肠炎菌)情况,为疾病防治工作提供科学依据。方法在敖汉旗长胜乡随机采集鼠类、家畜家禽粪便等样品,对小肠结肠炎菌进行分离、培养、鉴定并分型,用PCR方法进行毒力因子测定。结果 2011~2012年共检验各类样品381份,检出小肠结肠炎菌7株,2011年检出的7株为生物1A和3型,生物3型的2株菌均为O∶3血清型,均携带ail,ystA,yadA,virF,rfbc毒力基因。结论猪是致病性小肠结肠炎菌重要的携带者,敖汉旗境内的猪体内具有携带O∶3血清型小肠结肠炎菌。
Objective To understand the situation of Yersinia enterocolitica (hereinafter referred to as enterocolitis) in domestic mice and livestock in Aohan Banner, and provide a scientific basis for disease prevention and control. Methods Samples of rodent and livestock poultry were randomly collected from Changsheng Township, Aohan County. The enterocolitis bacteria were isolated, cultured, identified, and typed. PCR was used to determine virulence factors. Results A total of 381 samples of various types were tested from 2011 to 2012. Seven strains of enterocolitis were detected. Seven strains were identified as type 1A and type 3 in 2011, and two strains of type 3 were O: 3 serotype , All carrying ail, ystA, yadA, virF, rfbc virulence genes. Conclusion Pig is an important carrier of pathogenic enterocolitis bacteria, and pigs in Aohanqi harbor O-3 serotype enterocolitis.