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在前期应用抑制性消减杂交技术（ＳＳＨ）克隆肾癌相关新基因片段ＧＹＬＺ－ＲＣＣ１８的基础上，应用ＳＭＡＲＴ ＲＡＣＥ技术克隆ＧＹＬＺ－ＲＣＣ１８的全长约 ３．５ ｋｂ（ＧｅｎＢａｎｋ登录号： ＢＥ８２５１３３）；应用免疫荧光原位杂交技术进行染色体定位；应用逆转录ＰＣＲ的方法特异扩增其第１可读框区５０４ ｂｐ（碱基对）长序列片段，检测其在肾癌组织和细胞系中的表达特点，以及与肾癌组织分级、分期的关系．结果表明ＧＹＬＺ－ＲＣＣ１８定位于第１４号染色体末端，它在肾癌特异高表达，正常肾表达量极低甚至不表达，两者表达量差别在ｌ～９倍之间；ＧＹＬＺ－ＲＣＣ１８在高分级、高分期肾癌中表达明显高于低分级、低分期肾癌的表达．通过转染ＧＹＬＺ－ＲＣＣ１８的反义寡核苷酸阻断其表达，可明显抑制ＧＲＣ－１的生长、降低其增殖活性、减少核分裂、并诱导其持续性凋亡．研究表明ＧＹＬＺ－ＲＣＣ１８是与肾癌发生发展密切相关的重要癌基因，它的表达与肾癌细胞的生存、增殖、抗凋亡发展密切相关，它的成功克隆为阐明肾癌发生发展的分子生物学机制开辟了新的途径．
On the basis of GYLZ-RCC18, a novel gene fragment cloned by suppression subtractive hybridization (SSH), a total length of about 3.5 kb (GenBank accession no. BE825133) was cloned using SMART RACE technology. Immunofluorescence in situ hybridization was used to detect the chromosomal location. Reverse transcription PCR was used to amplify the 504 bp (base pair) long sequence in the first open reading frame to detect its expression in renal cell carcinoma and cell lines , As well as with renal cell carcinoma staging, staging relationship. The results showed that GYLZ-RCC18 located at the end of chromosome 14, it is specifically expressed in renal cell carcinoma, normal kidney expression is very low or not expressed, the difference between the two levels of 1 to 9 times; GYLZ-RCC18 in high-grade , High-stage renal cell carcinoma was significantly higher than low-grade, low-stage renal cell carcinoma expression. Antisense oligonucleotide transfection of GYLZ-RCC18 blocked the expression of GRC-1, significantly inhibited the growth of GRC-1, decreased its proliferation activity, reduced mitosis and induced its persistent apoptosis. Studies have shown that GYLZ-RCC18 is an important oncogene closely related to the development of renal cell carcinoma. Its expression is closely related to the survival, proliferation and anti-apoptosis of renal cell carcinoma. Its successful cloning is to clarify the molecular biology The mechanism has opened up new avenues.