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目的探讨PTEN基因启动子甲基化及其mRNA异常表达与膀胱移行细胞癌临床及病理特征的关系。方法应用甲基化特异性PCR(MSP)法检测膀胱移行细胞癌组织(48例)和正常膀胱组织(15例)PTEN基因启动子甲基化状态,应用RT-PCR技术检测其mRNA的表达水平。结果膀胱癌组织中PTEN启动子甲基化率为47.9%(23/48),而在15例正常膀胱组织中其启动子未发生甲基化,两组间差异具有统计学意义(P<0.01);PTEN基因mRNA在膀胱癌组织和正常膀胱组织中的阳性表达率分别是56.2%(27/48)和100.0%(15/15),两组间差异有统计学意义(P<0.01);PTEN基因启动子甲基化率与其mRNA表达水平在不同病理分级、临床分期间差异有统计学意义(P<0.05),且PTEN启动子甲基化与其mRNA表达存在明显关联性(χ2=21.372,r=0.555,P<0.01)。结论 PTEN基因启动子甲基化可导致其mRNA表达的缺失,对膀胱移行细胞癌的发生及转移有着极其重要的影响。
Objective To investigate the relationship between the methylation of PTEN gene promoter and the abnormal expression of PTEN gene and the clinical and pathological features of transitional cell carcinoma of the bladder. Methods Methylation-specific PCR (MSP) was used to detect the methylation status of PTEN promoter in bladder transitional cell carcinoma (48 cases) and normal bladder tissue (15 cases). The mRNA expression level of PTEN was detected by RT-PCR . Results The methylation rate of PTEN promoter was 47.9% (23/48) in bladder cancer tissues, but no methylation was found in 15 cases of normal bladder tissues, the difference was statistically significant (P <0.01) ). The positive expression rates of PTEN mRNA in bladder cancer tissues and normal bladder tissues were 56.2% (27/48) and 100.0% (15/15), respectively. There was significant difference between the two groups (P <0.01). The methylation rate of PTEN gene promoter and its mRNA expression level in different pathological grades and clinical stages were significantly different (P <0.05), and PTEN promoter methylation was significantly correlated with its mRNA expression (χ2 = 21.372, r = 0.555, P <0.01). Conclusions Methylation of PTEN gene promoter leads to the loss of its mRNA expression, which plays an important role in the genesis and metastasis of bladder transitional cell carcinoma.