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目的观察苯妥英钠(PHT)对内毒素作用下人牙周膜干细胞(h PDLSCs)在牙骨质片上附着的影响。方法本研究于2012年6月至2013年2月在滨州医学院中心实验室和西安交通大学医学院中心实验室完成。利用扫描电镜和透射电镜观察h PDLSCs分别在20μg/m L PHT+100 ng/m L脂多糖(LPS)与100 ng/m L LPS作用下在牙骨质片上的附着情况以及细胞超微结构的变化。结果与细胞培养液中加入100 ng/m L LPS比较,培养液中加入20μg/m L PHT+100 ng/m L LPS培养72 h后h PDLSCs胞浆内细胞器更丰富,粗面内质网扩张,高尔基复合体发达,有大量的线粒体和核糖体;培养第7 d和第21 d,细胞生长更密集,伪足较多且粗大,与牙骨质片的附着更紧密,细胞周围钙盐沉积以及胶原蛋白合成更加活跃。结论 PHT可促进h PDLSCs在牙骨片上的附着和生长。
Objective To observe the effect of phenytoin sodium (PHT) on the adhesion of h PDLSCs to the cementum after endotoxin treatment. Methods The study was performed in the Central Laboratory of Binzhou Medical College and the Central Laboratory of Xi’an Jiaotong University Medical College from June 2012 to February 2013. Scanning electron microscopy and transmission electron microscopy were used to observe the attachment of h PDLSCs on cementum in the presence of 20 μg / mL PHT plus 100 ng / mL lipopolysaccharide (LPS) and 100 ng / mL LPS, respectively. Variety. Results Compared with 100 ng / mL LPS in culture medium, h PDLSCs were more abundant in cytoplasm and expanded in endoplasmic reticulum by adding 20 μg / mL PHT + 100 ng / mL LPS for 72 h , The Golgi complex was developed with a large number of mitochondria and ribosomes. On the 7th day and the 21st day, the cells grew more densely with more pseudopodia and thicker, more closely attached to the cementum, and the deposition of calcium around the cells As well as collagen synthesis more active. Conclusion PHT can promote the attachment and growth of h PDLSCs on the dental pulp.