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目的研究超抗原金黄色葡萄球菌肠毒素B(staphylococcal enterotoxin B,SEB)诱导的耐受效应和耐受调节机制。方法收集SEB活化10天的细胞,充分洗涤后做为效应细胞,分别与刀豆蛋白(ConA)、脂多糖(LPS)和白介素-2(IL-2)共同培养,用MTT方法测定细胞的耐受性应答反应。正常淋巴细胞在与ConA、LPS、IL-2共同培养的同时添加效应细胞,用MTT方法测定效应细胞的抑制性应答反应功能。流式细胞技术解析耐受性效应细胞类型。结果效应细胞对ConA、LPS和IL-2的应答反应能力明显降低(P<0.01,n=3),但保持对ConA的应答反应能力。效应细胞抑制正常淋巴细胞与ConA、LPS和IL-2的应答反应(P<0.01,n=3),尤其抑制ConA和IL-2诱导的细胞增殖。SEB活化的效应细胞中CD8+NK1.1+、TcRVβ8+NK1.1+和CD4+NK1.1+NKT细胞以及TcRVβ8+T、CD8+T细胞数量明显增加(P<0.01和P<0.05,n=4)。结论超抗原SEB诱导的耐受性应答反应是效应细胞的直接作用,与细胞因子无关;这些效应细胞能抑制T淋巴细胞增殖,并保存识别ConA的受体功能。
Objective To investigate the tolerance and tolerance mechanism induced by superantigen staphylococcal enterotoxin B (SEB). Methods The cells that were activated by SEB for 10 days were collected and washed well as effector cells. The cells were co-cultured with ConA, LPS and IL-2, respectively. The cell resistance Accepted response response. Normal lymphocytes were cultured in the same culture with ConA, LPS and IL-2, while effector cells were added. The inhibitory response function of effector cells was measured by MTT assay. Flow cytometric analysis of resistant effector cell types. RESULTS: The responsiveness of effector cells to ConA, LPS and IL-2 was significantly decreased (P <0.01, n = 3), but their responsiveness to ConA was maintained. Effector cells inhibited the response of normal lymphocytes to ConA, LPS and IL-2 (P <0.01, n = 3), and in particular inhibited the proliferation of cells induced by ConA and IL-2. The numbers of CD8 + NK1.1 +, TcRVβ8 + NK1.1 + and CD4 + NK1.1 + NKT cells and TcRVβ8 + T and CD8 + T cells in SEB-activated effector cells were significantly increased (P <0.01 and P <0.05, respectively = 4). CONCLUSIONS: Superantigen SEB-induced tolerance response is a direct effect of effector cells and is independent of cytokines. These effector cells can inhibit T lymphocyte proliferation and preserve the function of recognizing ConA receptors.