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目的探讨小儿再生障碍性贫血骨髓细胞的增殖活性。方法收集正常骨髓８例，再生障碍性贫血１９例，每例取２～３张骨髓涂片以胶质银技术进行ＡｇＮＯＲ染色。油镜下依细胞系列每类细胞计数８～１００个细胞核内的核仁形成区蛋白（ＡｇＮＯＲ）的颗粒数，求均值，以ｔ检验进行统计学处理。结果急慢性再障骨髓细胞ＡｇＮＯＲ均值显著低于正常骨髓组，依次以巨核、早中幼红、早中幼粒细胞下降最明显（Ｐ＜０．００１，Ｐ＜０．０５）。急慢性再障之间ＡｇＮＯＲ均值也有显著性差异。单核及淋巴细胞无改变。结论ＡｇＮＯＲ技术对小儿再生障碍性贫血的诊断、疗效判定及骨髓细胞增殖障碍机制的探讨有一定价值。
Objective To investigate the proliferative activity of pediatric aplastic anemia bone marrow cells. Methods Eight cases of normal bone marrow and 19 cases of aplastic anemia were collected. Two to three bone marrow smears were taken in each case. AgNOR staining was performed with colloidal silver technique. Under the microscope, the number of nucleolar organizer nucleolar organizer protein (AgNOR) was counted in each type of cells according to cell series, and the average value was calculated. The statistical analysis was performed by t-test. Results The mean AgNOR of acute and chronic aplastic anemia was significantly lower than that of normal bone marrow, followed by megakaryocytes, early-middle-aged erythema and early-neutrophil (P <0.001, P <0.05). There was also a significant difference in mean AgNOR between acute and chronic aplasia. Monocytes and lymphocytes did not change. Conclusion The AgNOR technique has some value in the diagnosis of children with aplastic anemia, the determination of therapeutic effect and the mechanism of bone marrow cell proliferative disorders.