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目的:筛选出复方三黄胶囊抑制肿瘤血管生成的活性部位。方法:采用鸡胚绒毛尿囊膜(chorioallantoic mem-brane,CAM)法进行在体血管抑制实验。结果:①7 d时,将钴60照射过的超微粉碎的复方三黄胶囊粉用不同浓度的乙醇混悬,用20μm的移液枪加到直径5 mm大的紫外线照射的明胶海绵2 d后,明胶海棉周围出现明显的、似“轮辐状”的微细毛细血管,血管数量少于生理盐水对照组(P<0.05),紫杉醇阳性对照组与生理盐水空白对照组没有显著性差异。②将同样处理过的复方三黄胶囊粉用乙酸乙酯提取,再用pH梯度法萃取过的不同部位,做同样的鸡胚绒毛尿囊膜药理实验,明胶海绵周围走行的辐射微血管明显减少,与生理盐水对照组比较(P<0.05),苏拉明阳性对照组与生理盐水空白对照组有显著差异(P<0.01)。结论:对比2种不同的提取方式和选用2种不同阳性对照药,乙酸乙酯不同萃取部位的药理实验结果重复性好,实验操作难度小,苏拉明阳性对照组的结果比较稳定(P<0.05)。选择乙酸乙酯提取部位和苏拉明做阳性对照具有可操作性和可行性。
Objective: To screen out the active part of compound Sanhuang Capsule in inhibiting the tumor angiogenesis. Methods: In vivo vasoconstriction assay was performed using chorioallantoic mem-brane (CAM) method. RESULTS: On day 7, ultrafine crushed compound Sanhuang capsule irradiated with Cobalt 60 was suspended in ethanol with different concentrations. After a 5-mm diameter UV-irradiated gelatin sponge was applied with a 20μm pipette for 2 days (P <0.05). There was no significant difference between the paclitaxel-positive control group and the saline control group. There was obvious micro-capillaries around the gelatin sponge like “spokes ”. ② The same treated compound Sanhuang capsule powder extracted with ethyl acetate, and then pH gradient extraction of different parts of the chick chorioallantoic membrane to do the same pharmacological experiments, gelatin sponge walking around the radiation microvascular significantly reduced, Compared with saline control group (P <0.05), there was significant difference between sulamin positive control group and normal saline control group (P <0.01). CONCLUSION: Compared with 2 different extraction methods and 2 different positive control drugs, the results of pharmacological experiments with different extraction fractions of ethyl acetate have good repeatability, little difficulty in experimental operation and relatively stable results in suramin positive control group (P < 0.05). Select ethyl acetate extract and suramin as a positive control with maneuverability and feasibility.