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目的 探讨白细胞介素 (IL) 10启动子区近端SNPs( 10 82 / 819/ 5 92 )多态性对IL 10mRNA表达水平影响 ,从而明确该多态性对儿童系统性红斑狼疮 (SLE)发病的影响。方法 6 5例儿童SLE及家系中应用TaqmanPCR技术定量检测患儿及父母IL 10mRNA基础表达水平 ,结合此前曾对该样本进行的IL 10启动子区近端单核苷酸多态性研究结果 ,分析各单倍型尤其是GCC和ATA对IL 10mRNA表达水平影响。从另一个角度研判IL 10启动子区近端SNPs多态性与儿童SLE发病有无内在联系。结果 患儿中单倍型 10 82 G 819 C (GCC)显示与IL 10高分泌有关。结论 儿童SLE中单倍型GCC可能与IL 10高分泌有关 ,但不具备独立的调控作用 ,极有可能组合另一SLE易感基因共同调节IL 10高分泌从而影响儿童SLE发病。
Objective To investigate the effects of polymorphisms of SNPs (10 82/819/5 92) in IL-10 promoter region on the expression of IL 10 mRNA in order to clarify the role of this polymorphism in the pathogenesis of childhood systemic lupus erythematosus (SLE) Impact. Methods 65 cases of children with SLE and pedigree TaqmanPCR quantitative detection of children and their parents IL 10mRNA basic expression level, combined with the previous sample had IL10 promoter region proximal single nucleotide polymorphism study results, analysis The effects of haplotypes, especially GCC and ATA, on IL 10 mRNA expression levels. From another perspective to determine the IL 10 promoter SNP polymorphism and SN in children with or without intrinsic correlation. Results The haplotype 10 82 G 819 C (GCC) in children showed an association with IL 10 hypersecretion. Conclusion The haplotype GCC in children with SLE may be associated with IL 10 hypersecretion but not with independent regulation. It is highly possible that a combination of another SLE susceptible gene may regulate IL 10 hypersecretion and thus affect the incidence of SLE in children.