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目的探讨E-selectin(内皮细胞选择素)和ICAM-1(细胞间粘附分子-1)在肝癌细胞与血管内皮细胞黏附中的作用:筛选有临床应用前景、能预防肝癌复发与转移的抗黏附药物。方法实时荧光定量PCR检测E-selectin和ICAM-1mRNA在肝静脉内皮细胞ED25和脐静脉内皮TNFα刺激后细胞ECV304上的表达;应用体外粘附实验检测肝癌细胞HepG2与活化的ED25和ECV304的粘附,并检测不同药物对抗粘附作用的影响。结果 E-selectin和ICAM-1可高表达于活化后的ED25和ECV304;且能介导HepG2与ED25和ECV304的粘附;地塞米松、丹参酮ⅡA有较强的抑制HepG2与ED25粘附的作用。结论 E-selectin和ICAM-1可促进肝癌细胞与内皮细胞的粘附;地塞米松、丹参酮ⅡA可起到抗肝癌细胞与血管内皮细胞粘附的作用。
Objective To investigate the role of E-selectin (ICAM-1) and ICAM-1 in the adhesion of hepatocellular carcinoma cells to vascular endothelial cells: Screening has clinical application prospect and can prevent the recurrence and metastasis of liver cancer Adhesion drugs. Methods Real-time quantitative PCR was used to detect the expression of E-selectin and ICAM-1 mRNA on ECV304 cells stimulated by TNFα in hepatic vein endothelial cells (ED25) and human umbilical vein endothelium (TNFα). The adhesion of HepG2 cells to activated ED25 and ECV304 cells , And test the effect of different drugs on the adhesion. Results E-selectin and ICAM-1 were highly expressed in activated ED25 and ECV304, and could mediate the adhesion of HepG2 to ED25 and ECV304. Dexamethasone and tanshinone ⅡA inhibited the adhesion of HepG2 to ED25 . Conclusion E-selectin and ICAM-1 can promote the adhesion of hepatocellular carcinoma cells and endothelial cells. Dexamethasone and tanshinoneⅡA can inhibit the adhesion of hepatocellular carcinoma cells and vascular endothelial cells.