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目的比较3种检测方法对肺结核患者痰菌检出率,探索噬菌体生物扩增法提高肺结核病患者结核菌阳性检出率的可行性。方法对同1例肺结核患者的痰标本,采用直接厚涂片法、结核分枝杆菌培养法和噬菌体生物扩增法检测结核分枝杆菌。结果在150例肺结核患者痰标本中,直接厚涂片法检出菌阳26例(检出率为17.3%),痰结核分枝杆菌培养阳性33例(检出率为22.0%),噬菌体生物扩增法阳性85例(检出率为56.7%),噬菌体扩增法阳性检出率高于直接厚涂片法和分枝杆菌培养法(χ2=51.8,38.8,P<0.01)。结论噬菌体生物扩增法可提高肺结核病例的痰菌检出率。
Objective To compare the detection rate of sputum bacteria in three kinds of detection methods in patients with pulmonary tuberculosis and to explore the feasibility of using phage amplification method to increase the positive detection rate of tuberculosis in patients with pulmonary tuberculosis. Methods Mycobacterium tuberculosis was detected by direct thick smear method, Mycobacterium tuberculosis culture method and phage biological amplification method on sputum samples from 1 pulmonary tuberculosis patients. Results In 150 sputum specimens from patients with pulmonary tuberculosis, 26 cases of positive bacilli (detection rate was 17.3%) were detected by direct thick smear method, 33 cases were positive for sputum Mycobacterium tuberculosis culture (detection rate was 22.0%), 85 were positive (56.7%). The positive rate of phage amplification was higher than that of direct smear and mycobacterium culture (χ2 = 51.8, 38.8, P <0.01). Conclusion The phage amplification method can improve the detection rate of sputum bacteria in pulmonary tuberculosis cases.