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目的比较牛鲍氏计数板的传统计数方法和新计数方法对细胞计数结果的影响。方法用传统计数方法和新计数方法计数118份抗凝血标本中的红细胞,然后将结果与标准值对照。结果在109份有效样本中,有57份(52.29%)用新方法计数结果更准确,40份(36.70%)用传统方法计数结果更准确,12份(11.01%)用两种方法计数引出的误差相同。新方法计数误差的平均值为(0.071±0.005)×1012/L,明显低于传统方法计数误差的平均值(0.079±0.006)×1012/L,差异有统计学意义(P<0.01)。结论采用新方法在减小牛鲍氏计数板对细胞计数的误差方面可能有一定的作用。
OBJECTIVE: To compare the effects of the traditional counting method and the new counting method on the cell counting results of cattle abalone counting plate. Methods The erythrocytes in 118 anticoagulated blood samples were counted by the traditional counting method and the new counting method, and the results were compared with the standard value. RESULTS Of the 109 valid samples, 57 (52.29%) were more accurately counted by the new method, 40 (36.70%) were more accurate by the traditional method and 12 (11.01%) were counted by both methods The same error. The average error of the new method was (0.071 ± 0.005) × 1012 / L, which was significantly lower than that of the traditional method (0.079 ± 0.006) × 1012 / L, the difference was statistically significant (P <0.01). Conclusions The new method may play a role in reducing the error of cell counting by cattle abalone counting plate.