从患有腹水病的牙鲆体内和体表分离纯化菌株,经人工感染实验,证实分离的病原菌为腹水病的致病菌,通过形态学和理化特性研究,确定该病原菌为迟钝爱德华氏菌。用较强毒力的菌株制作福尔马林灭活疫苗,对牙鲆进行3种途径免疫接种,测定血清凝集抗体效价和免疫保护力,3种免疫途径中以腹腔注射效果最佳,免疫保护力达88%。 Isolation and purification of strains from the body and the surface of flounder with ascites disease, artificial infection experiments confirmed that the isolated pathogenic bacteria as the pathogens of ascites, morphological and physical and chemical properties of the study to determine the pathogenic bacteria is retarded Edwards. The virulent strain was used to make formalin inactivated vaccine, and the flounder was immunized in three ways. The serum agglutination antibody titers and immunoprotective power were measured. The intraperitoneal injection of the three immunization routes was the best, Protection of 88%.