Human telomeric G-quadruplex plays a crucial role in regulating the genome stability.Despite extensive studies on structures and kinetics of monomeric G-quadruplex,the interaction between G-quadruplexes is still in debate.In this work,we employ magnetic tweezers to investigate the folding and unfolding kinetics of two contiguous G-quadruplexes in 100-mM K+ buffer.The interaction between G-quadruplexes and the consequent effect on the kinetics of G-quadruplex are revealed.The linker sequence between G-quadruplexes is further found to play an important role in the interaction between two G-quadruplexes.Our results provide a high-resolution insight into kinetics of multimeric G-quadruplexes and genome stability.