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采用在实际工作中所遇到的脑挫伤案例标本 ,利用免疫组织化学染色 ,观察人脑挫伤后GFAP ,PCNA的改变 ,用图象分析仪对免疫组织化学染色阳性细胞的面积和灰度等进行定量测量 ,EpiInfo统计软件分析 ,得出人脑挫伤后GFAP ,PCNA变化的时间性规律 ,为脑挫伤形成时间推断 ,提供新的形态学依据。GFAP阳性细胞面积、灰度伤后24h即有显著增加 (P<0 01) ,7天达高峰 (P<0 01) ,以后有逐渐下降的趋势 ;PCNA阳性细胞面积、灰度于伤后4天达到最大值 (P<0 01) ,以后逐渐下降。因此 ,伤后人脑组织GFAP、PCNA免疫组织化学染色阳性细胞面积、灰度改变有时间规律 ,GFAP、PCNA可作为2天到20天间不同时间脑损伤的推断指标。
Using the specimens of brain contusion encountered in practical work, the changes of GFAP and PCNA after human brain contusion were observed by immunohistochemical staining. The area and gray scale of immunohistochemical staining positive cells were analyzed by image analyzer Quantitative measurement, EpiInfo statistical software analysis, obtained after human brain contusion GFAP, PCNA changes in the temporal regularity, inferred for the time of the formation of cerebral contusion, to provide a new morphological basis. The area of GFAP positive cells increased significantly (P <0.01) at 24h after gray-scale injury and reached a peak at 7d (P <0.01), and then gradually decreased. The area of PCNA positive cells, Day reached its maximum (P <0.01), then gradually decreased. Therefore, GFAP and PCNA immunohistochemical staining positive cell area and gray change have a regular pattern after injury, GFAP, PCNA can be used as inference indicators of brain injury at different time from 2 days to 20 days.