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目的:探讨白藜芦醇(RV)对鼻咽癌细胞CNE-1的放射增敏作用及其机制。方法:将实验分为空白对照组、单纯用药组(10、20和50μmol/L RV)、单纯照射组(1、2、3、4、5和7 Gy)以及实验组(药物+照射)。利用多靶单击模型拟合放射剂量-细胞生存曲线,检测RV对鼻咽癌细胞CNE-1的放射增敏效应。用流式细胞仪观察RV对细胞周期分布和细胞凋亡的影响,并观察细胞的形态学变化。结果:RV作用48 h后,10、20、50μmol/L RV的放射增敏比分别为1.07、1.32和1.66,呈药物浓度依赖性。流式细胞仪检测显示,RV和照射均可使G2/M期细胞阻滞,凋亡值(AI)增加,单纯用药组(10、20和50μmol/L RV)、单纯照射组(1、2、3、4、5和7 Gy)和实验组(药物+照射)的G2/M及AI值均随药物浓度的增加而增加,P值均<0.01。形态学检测可见凋亡小体,且两者具有协同作用。结论:RV对人鼻咽癌细胞CNE-1具有放射增敏效应,其机制可能与RV抑制细胞修复、导致G2/M期细胞阻滞和诱导细胞凋亡有关。
Objective: To investigate the radiosensitization effect of resveratrol (RV) on nasopharyngeal carcinoma cell line CNE-1 and its mechanism. Methods: The experiment was divided into blank control group, simple drug group (10, 20 and 50μmol / L RV), simple irradiation group (1,2,3,4,5 and 7 Gy) and experimental group (drug + irradiation). Radiosensitization effect of RV on nasopharyngeal carcinoma cell line CNE-1 was tested by fitting the radiation dose-cell survival curve with multi-target click model. The effects of RV on cell cycle distribution and apoptosis were observed by flow cytometry, and morphological changes of cells were observed. RESULTS: After 48 h of RV treatment, the radiosensitivities of 10, 20 and 50 μmol / L RV were 1.07, 1.32 and 1.66, respectively, in a drug concentration-dependent manner. Flow cytometry showed that both RV and irradiation could increase cell cycle arrest and AI in G2 / M phase, , 3, 4, 5 and 7 Gy) and G2 / M and AI of experimental group (drug + irradiation) both increased with the increase of drug concentration, P <0.01. Morphological examination revealed apoptotic bodies, and both had synergistic effects. CONCLUSION: RV has a radiosensitizing effect on human nasopharyngeal carcinoma cell line CNE-1, which may be related to the inhibition of cell repair by RV and the induction of cell cycle arrest at G2 / M stage and induction of cell apoptosis.