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目的 探讨松果体调节昼夜节律的作用机制。方法 在持续黑暗条件下 ,2 4h记录大鼠的踏转轮运动 ,观察其昼夜节律性变化 ,同时用免疫细胞化学方法 ,检测视交叉上核 (SCN)中光诱导的Fos蛋白的表达。结果 摘除松果体后 ,踏转轮运动白天少黑夜多的昼夜节律消失 ,Fos蛋白表达减少 ;补充褪黑素后能使之恢复。褪黑素 1(ML 1)受体拮抗剂Luzindole也能使踏转轮运动的昼夜节律消失和Fos蛋白表达减少。而ML 2受体拮抗剂Prazosin则不能。结论 松果体通过分泌褪黑素作用于SCN中的ML 1受体 ,从而调节踏转轮运动的昼夜节律性
Objective To explore the mechanism of pineal body regulating circadian rhythm. Methods Under continuous darkness, treadmill movement was recorded at 24 hours and the circadian rhythmicity was observed. Meanwhile, the light-induced Fos protein expression in the suprachiasmatic nucleus (SCN) was detected by immunocytochemistry. Results After removal of pineal gland, treadmill movement day and night rhythm disappear more and less night, Fos protein expression decreased; add melatonin can make it recover. Luzindole, a melatonin 1 (ML1) receptor antagonist, also decreases the circadian rhythm of treadmill exercise and decreases Fos protein expression. The ML2 receptor antagonist Prazosin can not. Conclusion The pineal gland modulates the circadian rhythm of treadmill exercise by acting on ML 1 receptors in SCN through the secretion of melatonin