为了解我院消毒液使用过程中的污染情况,特采样进行了检测。检测时,吸取中层消毒液1 ml,加至装有9ml含有相应中和剂的试管内。中和后,吸取混合液滴种于营养琼脂平板上,每平板滴10滴,每滴20μl。经在37℃培养72小时观察结果。细菌总数超过250 cfu/ml者为不合格。调查结果,总不合格率为40.91％(45/110)。其中,2.5％来苏儿溶液的不合格率为61.90％(13/21),最高;其次为75％乙醇 In order to understand the use of disinfectant in our hospital during the pollution, special sampling was tested. Test, draw medium disinfectant 1 ml, add to contain 9ml containing the appropriate neutralizer test tube. After neutralization, the mixture was drawn on a nutrient agar plate, dropping 10 drops per plate, 20 μl per drop. After 72 hours of incubation at 37 ° C, the results were observed. The total number of bacteria over 250 cfu / ml who failed. The survey results, the total failed rate was 40.91% (45/110). Among them, 2.5% to the Soviet Union Sol solution failed rate of 61.90% (13/21), the highest; followed by 75% ethanol