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应用图像细胞分析技术对外Ｎ－甲基－Ｎ－亚硝基脲（ＭＮＵ）建立的小鼠大肠癌模型进行细胞ＤＮＡ含量动态定量观察。正常成年小鼠结肠上皮以二倍体细胞（２Ｃ）为主。在ＭＮＵ作用下，不典型增生期出现４Ｃ细胞持续性扩增，并取代２Ｃ细胞成为主要的细胞群体；处于早期癌变时期仍主要以４Ｃ细胞为主要群体，但已开始出现异倍体（ＡＮ）干系的增殖；进入演进期后又出现ＡＮ干系的选择和优势性生长。研究表明在整个诱导癌变过程中大肠上皮细胞群体的ＤＮＡ干系主要呈现２Ｃ→４Ｃ→ＡＮ的选择过程，早期大肠癌以细胞ＤＮＡ含量４Ｃ为重要特征，而演进期大肠癌以ＤＮＡ含量ＡＮ为主要标志，为大肠癌发生的多阶段理论提供了实验依据。
The cellular DNA content was dynamically quantified in mouse colon cancer models established by external N-methyl-N-nitrosourea (MNU) using image cytometry. The normal adult mouse colonic epithelium is dominated by diploid cells (2C). Under the action of MNU, 4C cells continued to expand during the atypical hyperplasia, and replaced the 2C cells as the main cell population; in the early stage of canceration, 4C cells were still the main group, but aneuploidy (AN) has begun to appear. Proliferation of stem lines; after entering the evolutionary phase, the selection and superiority of the stemline of the AN have emerged. Studies have shown that during the entire process of inducing cancer, the DNA backbone of the epithelial cell population mainly presents the selection process of 2C→4C→AN. In early colorectal cancer, the 4C DNA content is an important feature, and the colorectal cancer is the main marker of the progressive colorectal cancer. It provides experimental basis for the multistage theory of colorectal cancer.