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目的研究结直肠癌患者肠道黏膜相关菌群组成差异,探索肠道菌群在结直肠癌发生发展中的作用。方法用末端限制片段长度多态性(Terminal restriction fragment length polymorphism,T-RFLP)技术分析50例结直肠癌患者癌组织、癌旁正常黏膜与健康对照组肠道黏膜相关细菌组成差异。结果与健康对照组相比,结直肠癌患者肠道黏膜相关细菌丰度显著增加(P<0.05),多样性显著降低(P<0.05)。结直肠癌患者癌组织与癌旁正常黏膜的黏膜相关细菌组成相近,但与健康对照组存在显著差异。MspI酶切的160bp、560bp的T-RF片段在结直肠癌患者癌组织及癌旁正常黏膜中为优势片段,而在健康对照组中缺失。相反,MspI酶切的66bp、74bp、141bp的T-RF片段在健康对照组为优势片段,但在结直肠癌癌组织及癌旁正常黏膜中缺失。结论肠道菌群失调与结直肠癌的发生发展密切相关。MspI酶切的66bp、74bp、141bp、160bp、560bp的T-RF片段所代表的细菌可能在结直肠癌的发生发展中起重要作用。
Objective To study the composition of intestinal mucosa-associated bacterial flora in patients with colorectal cancer and explore the role of intestinal flora in the development of colorectal cancer. Methods Thirty-nine patients with colorectal cancer were analyzed by using the terminal restriction fragment length polymorphism (T-RFLP) technique to analyze the differences in the bacterial composition of intestinal mucosa between 50 cases of colorectal cancer tissues, adjacent normal mucosa and healthy controls. Results Compared with the healthy control group, the bacterial abundance in intestinal mucosa of patients with colorectal cancer was significantly increased (P<0.05), and the diversity was significantly reduced (P<0.05). The mucosal-associated bacteria in colorectal cancer patients were similar to those in adjacent normal mucosa, but there were significant differences with healthy controls. The 160 bp and 560 bp T-RF fragment excised by MspI was the predominant segment in the colorectal cancer tissue and adjacent normal mucosa, but was absent in the healthy control group. On the contrary, the 66-bp, 74-bp, and 141-bp T-RF fragments digested by MspI were dominant in healthy controls, but were absent in colorectal cancer tissues and adjacent normal mucosa. Conclusion The imbalance of intestinal flora is closely related to the occurrence and development of colorectal cancer. The bacteria represented by MspI digested 66-bp, 74-bp, 141-bp, 160-bp, and 560-bp T-RF fragments may play an important role in the development of colorectal cancer.