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1983年10月从3日龄陆地棉(Gossypiumhirsutum)栽培品种C312,RQSX-1-1,C3110,P784,1303,T25和T169幼苗下胚轴产生的6～8周愈伤组织发现含有球状到心形早期胚,以与陆地棉胚珠的胚显微镜观察比较为准。愈伤组织放入液体培养基,其中T25和C3112形成胚胎发生的细胞悬浮体。C312选择为胚发育成成熟植株的典型体系。愈伤组织是从MS 盐类附加100mg/l 肌醇,10mg/l 盐酸硫胺素,1mg/l 菸酸,1mg/l 吡哆醇,30g/l 蔗糖,0.1mg/l 2,4-D 和0.1mg/l 激动素,或0.5mg/l 激动素。装有50ml液体培养基的欧氏三角瓶,接种约0.5g 愈伤组织,旋转振荡120rpm°,培养体经纱布过滤保持,取部分残留物培养在基础培养基上。含有单细胞和小细胞团(10～20细胞)的滤过物,转移到装有基础培养基的瓶内,基础培养基不加荷尔蒙,加谷酰胺,供发育
October 1983 The 6-8 week callus from hypocotyls of 3-day-old Gossypium hirsutum cultivars C312, RQSX-1-1, C3110, P784, 1303, T25 and T169 was found to contain globular to heart Shaped early embryos, with the embryos of cotton embryos observed and compared embryos prevail. Callus was placed in liquid medium, where T25 and C3112 formed embryogenic cell suspensions. C312 is a typical system for embryo development into mature plants. Callus was obtained from MS salts plus 100 mg / l myo-inositol, 10 mg / l thiamine HCl, 1 mg / l nicotinic acid, 1 mg / l pyridoxine, 30 g / l sucrose, 0.1 mg / l 2,4-D And 0.1 mg / l kinetin, or 0.5 mg / l kinetin. The Euclid containing 50 ml of liquid medium was inoculated with about 0.5 g of callus, rotated and swirled at 120 rpm °, the culture was filtered through a gauze, and a part of the residue was cultured on basal medium. The filtrate, containing single and small cell clusters (10-20 cells), is transferred to a flask containing basal medium supplemented with no hormones and supplemented with glutamine