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目的:探讨固相微萃取(SPME)与超临界流体萃取(SFE)所得南阳艾叶挥发油成分的不同,确定其主含成分及含量,并对其进行分析。方法:分别以SPME与SFE提取艾叶挥发油并以GC-MS进行对比分析。结果:以SPME法得到了38种化合物,SFE得到了26种化合物,GC-MS分析分别占其总量的95.17%和95.26%;通过对比质谱数据库(NIST,NBS)进行鉴别,前者鉴别出35个组分,后者鉴别出25个;各组分含量均不相同,但最高者均为1,8-桉树脑(分别为13.75%,18.26%),其他在SPME中依次为2-莰醇(11.58%)、异蒿属(甲)酮(7.15%)及对称二甲基脲(5.12%);在SFE中依次为异蒿属(甲)酮(12.15%),2-莰醇(11.38%)及反-罗勒烯(7.03%)。结论:SPME萃取与SFE萃取所得的艾叶挥发油成分差异较大,相较而言SPME具有较明显的优越性。
OBJECTIVE: To investigate the difference of the volatile oil components of the leaves of the Nanyang Aiye obtained by SPME and SFE, determine their main components and their contents, and analyze them. Methods: Volatile oil from leaves of Artemisiae Argyi was extracted by SPME and SFE respectively and compared by GC-MS. Results: Thirty-eight compounds were obtained by SPME method and 26 compounds were obtained by SFE method. GC-MS analysis accounted for 95.17% and 95.26% of their total amount, respectively. By the comparison of mass spectra database (NIST), the former identified 35 The latter identified 25; the content of each component was different, but the highest ones were 1,8-eucalyptol (13.75%, 18.26%, respectively), others were SPME followed by 2- (11.58%), artemisia (7.15%) and symmetrical dimethylurea (5.12%), followed by SFE in the order of 12.15%, 2-alcohol %) And trans-ocimene (7.03%). Conclusion: There are significant differences in the constituents of the volatile oil from the leaves extracted by SPME and SFE. In contrast, SPME has more obvious advantages.