论文部分内容阅读
目的研究携带 Panton-Valentine 杀白细胞素(Panton-Valentine leukocidin,PVL)基因金黄色葡萄球菌所致感染类型。方法利用多重 PCR 检测金黄色葡萄球菌临床分离株的 PVL 基因,应用多位点基因序列分型(multilocus sequence typing,MLST)技术对 PVL 基因阳性的菌株进行序列分型,耐甲氧西林金黄色葡萄球菌(methicillin-resistant Staphylococcus aureus,MRSA)的 SCCmec 基因分型采用多重 PCR。结果 26株携带 PVL 基因的金黄色葡萄球菌有13株为 ST88,5株为 ST239,5株为 ST398,ST25、ST30和 ST59各1株。20株为医院获得株,主要引起肺部感染和术后伤口化脓性感染;6株为社区感染株,主要引起软组织化脓性感染。7株 MSSA全部为 ST88,主要引起化脓性感染。而19株 MRSA分布在6种 ST 型中,主要有 ST88-SCCmecⅢA、ST239-SCCmecⅢ、ST398-SCCmecⅣ和ST398-SCCmecⅢ。26株菌株分布于14个病区,在产科发生过携带 PVL 基因的 ST88-SCCmecⅢA-MRSA 克隆株的播散。结论本院分离的携带 PVL 基因的金黄色葡萄球菌的序列型主要为 ST88、ST239 和 ST398,既可造成医院感染也可造成社区感染且可能引起克隆株的播散。
Objective To study the type of infection caused by Staphylococcus aureus carrying Panton-Valentine leukocidin (PVL) gene. Methods Multiplex PCR was used to detect the PVL gene in Staphylococcus aureus clinical isolates. The multilocus sequence typing (MLST) technique was used to sequence the PVL gene positive strains. The methicillin-resistant golden yellow grape SCCmec genotyping of methicillin-resistant Staphylococcus aureus (MRSA) using multiplex PCR. Results Staphylococcus aureus of 26 strains carrying PVL gene were ST88, 5 strains of ST239, 5 strains of ST398, 1 strain of ST25, ST30 and ST59 respectively. Twenty strains were hospital-acquired strains, mainly causing pulmonary infections and postoperative wound suppurative infections; 6 were community-acquired strains that caused mainly soft tissue suppurative infections. All 7 strains of MSSA were ST88, causing mainly purulent infection. The 19 strains of MRSA were distributed in 6 ST types, including ST88-SCCmecⅢA, ST239-SCCmecⅢ, ST398-SCCmecⅣ and ST398-SCCmecⅢ. Twenty-six strains were distributed in 14 wards and disseminated in the obstetric department of the ST88-SCCmec III A-MRSA clone carrying the PVL gene. Conclusions The sequences of the Staphylococcus aureus isolated from our hospital with PVL gene are mainly ST88, ST239 and ST398, which can cause nosocomial infection and cause community infection and may cause the spread of clonal strains.