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芽胞的离心洗涤是芽胞悬液制备过程中的一个重要步骤,其目的是去除其中所含培养基的残存物质及细胞的裂解物质,以得到较纯净的芽胞悬液。试验证明,经下述离心洗涤的芽胞,于冰箱中保存半年以上仍很稳定。芽胞的离心洗涤包括以下三个步骤:①第一阶段离心,主要是去除芽胞液中培养基的残存物质。将经过滤的芽胞液,在无菌条件下移入灭菌离心管中加盖连同套管放于天
Centrifugal washing of spores is an important step in the preparation of spore suspensions for the purpose of removing the remaining substances in the culture medium contained therein and the cell lysis material so as to obtain a relatively pure spore suspension. Test proved that the spores after washing by centrifugation, stored in the refrigerator for more than six months is still very stable. Centrifugal washing spores include the following three steps: ① the first stage of centrifugation, mainly to remove residual material in the culture medium of the spore. The filtered spore fluid, sterile conditions into the sterile centrifuge tube capped together with the casing on the day