采用吖啶橙(AO)与溴化乙啶(EB)荧光双染色以及Hoechst 33258荧光染色法对HMC毒素诱导玉米B37-C、B37-N根冠细胞凋亡率进行检测。结果表明,采用AO/EB双染色后,当HMC毒素浓度为50、100、150μg/mL,处理7 h时B37-C根冠细胞凋亡率分别为24.8%、58.2%、70.2%,B37-N仅为11.0%、25.7%和36.6%;当浓度为150μg/mL,处理时间4 h时B37-C根冠细胞最大凋亡率为62.3%,B37-N为25.8%。经Hoechst 33258染色后,HMC毒素浓度为50、100、150μg/mL,处理7 h时B37-C的根冠细胞凋亡率分别为32.5%、58.7%、74.5%,B37-N仅为7.5%、22.3%和30.7%;HMC毒素浓度为150μg/mL,处理4 h时B37-C的细胞凋亡率为62.3%,B37-N为19.3%。 Apoptosis rates of corn B37-C and B37-N induced by HMC toxin were detected by fluorescent double staining of acridine orange (AO) and ethidium bromide (EB) and Hoechst 33258 fluorescence staining. The results showed that the apoptotic rates of B37-C cells were 24.8%, 58.2% and 70.2%, respectively, at the concentrations of HMC to 50, 100 and 150μg / mL for 7 h with AO / EB double staining. N was only 11.0%, 25.7% and 36.6%, respectively. The maximum apoptosis rate of B37-C cells was 62.3% and 25.8% at the concentration of 150μg / mL for 4 hours. After Hoechst 33258 staining, the HMC toxin concentrations were 50, 100 and 150μg / mL. The apoptosis rates of B37-C cells were 32.5%, 58.7%, 74.5% and 737% , 22.3% and 30.7% respectively. The HMC toxin concentration was 150μg / mL. The apoptosis rate of B37-C was 62.3% and B37-N was 19.3% at 4 h.