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目的 研究益气活血方药抑制大鼠肝星状细胞 (HSC)体外增殖的作用机理和有效部位群。方法 用脂多糖 (lipopolysccharide ,LPS)刺激体外培养的HSC增殖 ,同时加入不同时相的含药血清Scl、Sc2或正常鼠血清 (Sn)或该方药不同提取物 ,分别培养 2 4、4 8、72h ,MTT法测定细胞增殖率 ,流式细胞仪测定HSC凋亡率。结果 在培养各时相Sc1和Sc2对LPS刺激的HSC增殖都有明显的抑制作用 (P <0 0 5或P <0 0 1)。流式细胞仪测定HSC凋亡的结果显示Sc2明显促进了HSC的凋亡 (P <0 0 1)。该方药不同提取物以不同的模式抑制体外培养的大鼠HSC增殖。结论 益气活血方药含药血清具有抑制体外培养大鼠HSC增殖作用 ,并促进其凋亡 ;益气活血方药有不同的有效部位群 ,并以不同的模式发挥作用
Objective To study the mechanism of action of Yiqi Huoxue Recipe on proliferation of rat hepatic stellate cells (HSCs) in vitro and their effective site groups. Methods The lipopolysaccharide (lipopolysccharide, LPS) was used to stimulate the proliferation of HSC cultured in vitro. Simultaneously, different phase of drug-containing serum Scl, Sc2 or normal mouse serum (Sn) or different extracts of the drug were added to culture the HSCs. 72h, MTT assay cell proliferation rate, flow cytometry apoptotic rate of HSC. Results Sc1 and Sc2 significantly inhibited the proliferation of LPS-stimulated HSCs in culture (P <0 05 or P <0 01). Flow cytometry assay of HSC apoptosis revealed that Sc2 significantly promoted HSC apoptosis (P < 0.01). Different extracts of this formula inhibited proliferation of rat HSC cultured in vitro in different modes. Conclusion Yiqihuoxue prescription drug-containing serum inhibits the proliferation of rat HSC in vitro and promotes its apoptosis; Yiqihuoxue prescription has different effective site groups and plays a role in different modes.