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目的:观察三七对酒精性肝病(ALD)大鼠肝组织转化生长因子-β(TGF-β)表达的影响。方法:110只雄性SD大鼠随机分为正常组30只,模型组35只,三七高剂量组、三七低剂量组和硫普罗宁组各15只,连续14周白酒-玉米油-吡唑混合液灌胃建立ALD模型,造模同时三七高、低剂量组和硫普罗宁组分别灌服1.2g/(kg体重.天)、0.6g/(kg体重.天)的三七粉及0.1g(kg体重.天)的硫普罗宁;4周末、8周末、14周末正常组分别随机处死大鼠10只,模型组分别随机处死大鼠8只用于肝脏病理变化的动态观察;14周末处死剩余所有大鼠,光镜观察肝组织脂肪变、炎症及纤维化程度,酶法测定血清ALT、AST水平,脂酶法测定血脂(TG、CHOL、HDL-C、LDL-C),免疫组化二步法检测肝组织TGF-β蛋白的表达。结果:与同期正常组相比,各时间点模型组大鼠肝组织脂肪变及炎症程度计分明显增高(P<0.01);8周末、14周末肝脏TGF-β表达明显增加(P<0.05);实验结束时模型组大鼠血清ALT、AST及CHOL、HDL-C水平较正常组明显增高(P<0.01)。三七高、低剂量组及硫普罗宁组大鼠肝组织脂肪变及炎症程度、血清ALT、AST水平和肝脏TGF-β蛋白表达较模型组明显减轻或降低(P<0.01或0.05),对血脂未见明显影响(P>0.05)。结论:用白酒-玉米油-吡唑混合液灌胃14周能成功复制大鼠ALD模型;三七可明显减轻ALD大鼠肝组织脂肪变和炎症程度,抑制ALD大鼠肝脏TGF-β蛋白的表达,后者可能是三七有效防治ALD的机制之一。
Objective: To observe the effect of Panax notoginseng on the expression of transforming growth factor-β (TGF-β) in liver tissue of alcoholic liver disease (ALD) rats. METHODS: One hundred and ten male SD rats were randomly divided into normal group (n=30), model group (n=35), panax notoginseng high-dose group, sanqi low-dose group, and tiopronin group (n=15). The ALD model was established by intragastric administration of azole mixture. At the same time, the Sanqi high and low dose group and tiopronin group were given 1.2g/(kg body weight. days) and 0.6g/(kg body weight. days) of Sanqi powder. Tiopronin was given at 0.1 g (kg body weight per day). Rats in the normal group were sacrificed at the end of the 4th, 8th, and 14th weekends. Ten rats in the model group were randomly killed and used for dynamic observation of liver pathological changes. All rats were sacrificed at the end of the 14th week. The degree of liver steatosis, inflammation and fibrosis were observed by light microscopy. Serum ALT and AST levels were measured by enzymatic method, and lipids (TG, CHOL, HDL-C, and LDL-C) were measured by lipase method. Two-step immunohistochemistry was used to detect the expression of TGF-β protein in liver tissue. Results: Compared with the normal group at the same time, the scores of steatosis and inflammation in the hepatic tissue of the model group were significantly higher at each time point (P<0.01); the expression of TGF-β was significantly increased at the 8th and 14th weekend (P<0.05). At the end of the experiment, the levels of serum ALT, AST, and CHOL and HDL-C in the model group were significantly higher than those in the normal group (P<0.01). The hepatic steatosis and inflammation, serum ALT, AST levels and hepatic TGF-β protein expression in the high and low dose groups and tiopronin group were significantly reduced or decreased compared with the model group (P<0.01 or 0.05). No significant effect of lipids was observed (P>0.05). Conclusion: The rat model of ALD can be successfully established by intragastric administration of liquor-corn oil-pyrazole mixture for 14 weeks. Panax notoginseng can significantly reduce the degree of liver steatosis and inflammation in ALD rats and inhibit the expression of TGF-β protein in liver of ALD rats. Expression, the latter may be one of the mechanisms of effective prevention and treatment of ALD.