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为评价卷烟烟气有害成分的细胞毒性及其联合作用,以人支气管上皮细胞(BEASe-2B细胞)为靶细胞,分别用卷烟烟气中5种有害成分4-甲基亚硝基-1-(3-吡啶基)-1-丁酮(NNK)、氨、苯并[a]芘、苯酚和巴豆醛单独及两两联合染毒,用MTT法测定染毒24h后的细胞存活率,计算细胞的平均半数致死剂量(LC50),用效应图解法对联合作用进行评价。结果显示:①NNK、氨、苯并[a]芘、苯酚和巴豆醛的LC50分别为87.2、395.5、5.4、61.9和6.9mg/L;②NNK与氨、NNK与苯并[a]芘、NNK与苯酚、NNK与巴豆醛、氨与苯并[a]芘、氨与苯酚、氨与巴豆醛、苯并[a]芘与苯酚、苯并[a]芘与巴豆醛、苯酚与巴豆醛的LC50分别为46.2、10.9、14.1、30.4、76.4、86.1、39.0、6.1、1.1和22.1mg/L;③NNK与氨、NNK与苯并[a]芘、NNK与苯酚、苯并[a]芘与苯酚、苯并[a]芘与巴豆醛、氨与巴豆醛存在明显的协同作用,而NNK与巴豆醛、苯并[a]芘与氨、苯酚与氨、苯酚与巴豆醛之间无协同作用。因此,卷烟烟气有害成分的细胞毒性存在明显差异,其联合作用的细胞毒性效应各不相同。
To evaluate the cytotoxicity of harmful components in cigarette smoke and their combined effects, human bronchial epithelial cells (BEASe-2B cells) were used as target cells. Five harmful components, 4-methylnitrosotoluene (3-pyridyl) -1-butanone (NNK), ammonia, benzo [a] pyrene, phenol and crotonaldehyde alone or in combination with each other. MTT assay was used to determine the cell viability after 24h exposure The average lethal dose of cells (LC50), the effect of graphic analysis of the joint effects were evaluated. The results showed that: (1) The LC50 of NNK, ammonia, benzo [a] pyrene, phenol and crotonaldehyde were 87.2, 395.5, 5.4, 61.9 and 6.9 mg / Phenol, NNK and crotonaldehyde, ammonia and benzo [a] pyrene, ammonia and phenol, ammonia and crotonaldehyde, benzo [a] pyrene and phenol, benzo [a] pyrene and crotonaldehyde, phenol and crotonaldehyde LC50 Respectively, 46.2,10.9,14.1,30.4,76.4,86.1,39.0,6.1,1.1 and 22.1mg / L; ③NKK and ammonia, NNK and benzo [a] pyrene, NNK and phenol, benzo [a] pyrene and phenol , Benzo [a] pyrene and crotonaldehyde, ammonia and crotonaldehyde have obvious synergistic effect, while there is no synergistic effect between NNK and crotonaldehyde, benzo [a] pyrene and ammonia, phenol and ammonia, phenol and crotonaldehyde. Therefore, the harmful components of cigarette smoke cytotoxicity there are significant differences, the combined effect of cytotoxic effects vary.