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本文通过采用可控酶解的方式酶解大豆蛋白获得了一种源自大豆的天冬氨酸蛋白酶抑制剂SAPI(soyaspartic proteinase inhibitor)。酶解液经过DEAE-52阴离子交换层析、Superdex Peptide 10/300 GL凝胶层析、SOURCE 15RPC反相层析分离后最终比抑制活力为254.2 IU/mg,纯化倍数为62。SAPI对胃蛋白酶(3000U)半数抑制浓度IC50为25.67μg/mL,有良好的热稳定性,属于一种非竞争性抑制剂。
In this paper, soybean aspartic protease inhibitor SAPI (soyaspartic proteinase inhibitor) was obtained by enzymatic hydrolysis of soy protein by controlled enzymolysis. After DEAE-52 anion exchange chromatography and Superdex Peptide 10/300 GL gel chromatography, the final specific inhibitory activity was 254.2 IU / mg and the purification fold was 62 after SOURCE 15RPC reverse phase chromatography. SAPI of pepsin (3000U) half-value concentration IC50 25.67μg / mL, has good thermal stability, is a non-competitive inhibitor.