本文以外来物质的极性为主,例举从酯或酰胺键的水解到芳香环的甲基化和仲胺的去甲基化二种代谢途径。而研究药物代谢,在于阐明外来药物的最终产物、测定生物体液,特别是血浆或血清内的代谢物,以选择合适的治疗剂量。目前,对这方面的研究常用一些经典的层析法(气相或薄层),但不足的是亲附于固定相的量随化合物极性的增加而增加。因此,对一些组份多、大部分是极性或在一定层析条件下可转为极性的生物样品,固定相污染就很快,使层析性能迅速下降。其次,化合物与代谢物所用的分析条件不同,代谢物亲水性较强,且许多均具有极性基团,会长时滞留 In this paper, the polarity of foreign substances mainly exemplified two metabolic pathways from hydrolysis of ester or amide bond to methylation of aromatic ring and demethylation of secondary amine. The study of drug metabolism, is to elucidate the final product of foreign drugs, biological fluids measured, especially in plasma or serum metabolites, to select the appropriate therapeutic dose. At present, some classical chromatographic methods (gas phase or thin layer) are often used in the research of this aspect, but the deficiency is that the amount of affinity to the stationary phase increases with the increase of the polarity of the compound. Therefore, for some biological samples with more components, most of them are polar or can be converted to polar under certain chromatographic conditions, the contamination of the stationary phase is rapid and the chromatographic performance is rapidly degraded. Second, the analytical conditions used for the compounds and metabolites are different, metabolites are more hydrophilic, and many have polar groups that persist for long periods