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目的探讨自体灭活T细胞免疫注射后诱导体内调节性T细胞(Treg)下调的机制。方法自体T细胞体外用刀豆蛋白(ConA)刺激活化,照射灭活T细胞给小鼠进行皮下和腹腔免疫(每只小鼠5×106/次),每隔5 d免疫一次,免疫3次后检测小鼠体内Treg的数量及功能;对照组小鼠皮下注射PBS。ELISA法检测血清中抗鼠CD25抗体。免疫小鼠血清分离后经尾静脉注入未免疫小鼠体内,检测受体小鼠Treg的数量及功能变化。结果免疫小鼠体内CD4+CD25+Foxp3+Treg数量较对照组减少(P<0.01),抑制功能也显著降低(P<0.01),但血清中抗CD25的抗体增加(P<0.01)。正常小鼠接受免疫小鼠血清后,Treg的数量和抑制功能下调(P<0.01)。结论采用ConA活化的自体灭活T细胞免疫,可诱导抗CD25抗体增加,进而减少体内CD4+CD25+Foxp3+Treg细胞数量和功能。
OBJECTIVE: To investigate the mechanism of in vivo regulation of regulatory T cells (Tregs) after autoinactivation of T cells. Methods Autologous T cells were stimulated with ConA in vitro. Mice were inoculated subcutaneously and intraperitoneally (5 × 106 / mouse) by irradiation of inactivated T cells, immunized every 5 d for 3 times After testing the number and function of Treg in mice; control mice were injected subcutaneously with PBS. Serum anti-mouse CD25 antibody was detected by ELISA. Immune mice were isolated from the tail vein and then injected into the uninjured mice to detect the number and function of Treg in recipient mice. Results The number of CD4 + CD25 + Foxp3 + Tregs in immunized mice was decreased (P <0.01) and the inhibitory function was also significantly decreased (P <0.01), but the serum anti-CD25 antibody was increased (P <0.01). The normal mice received immunized mice serum, the number of Treg and inhibitory function down (P <0.01). CONCLUSION: ConA-activated autologous inactivated T cells can induce an increase in anti-CD25 antibodies, thereby reducing the number and function of CD4 + CD25 + Foxp3 + Treg cells in vivo.