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目的:利用HL-1细胞建立快速起搏模型,对心房颤动(atrial fibrillation,AF)早期的重构现象进行初步研究。方法:培养HL-1细胞,建立快速电场刺激起搏细胞模型,利用全细胞膜片钳技术记录刺激前后HL-1细胞的动作电位周期,透射电镜观察细胞超微结构的变化。结果:将细胞接种于培养皿中,72 h后细胞呈融合状态,全细胞膜片钳记录培养HL-1细胞及经电场刺激(600次/min,1 V/cm)24 h后的心房肌细胞的动作电位周期,动作电位周期分别为106 ms,45 ms,刺激前后差异有统计学意义(P<0.05)。透射电镜观察到刺激后HL-1细胞超微结构发生去分化改变。结论:经快速起搏24 h后,HL-1细胞发生了电及结构重构;利用HL-1细胞建立快速起搏的房颤模型,可以对房颤早期的重构机制进行研究。
OBJECTIVE: To establish a rapid pacing model using HL-1 cells to study the early remodeling of atrial fibrillation (AF). Methods: HL-1 cells were cultured and paced cell model was established by rapid electric field stimulation. Whole-cell patch-clamp technique was used to record the action potentials of HL-1 cells before and after stimulation. The ultrastructural changes of HL-1 cells were observed by transmission electron microscopy. Results: The cells were seeded into culture dishes and the cells were confluent after 72 h. HL-1 cells were recorded by whole-cell patch-clamp and atrial myocytes were stimulated by electric field (600 V / cm, 1 V / cm) (P <0.05). The periods of action potentials were 106 ms and 45 ms respectively. There was significant difference between before and after stimulation (P <0.05). The ultrastructural changes of HL-1 cells were observed by transmission electron microscopy. CONCLUSION: Electrical and structural remodeling of HL-1 cells occurs after 24 h of rapid pacing. Establishment of rapid paced atrial fibrillation model using HL-1 cells can study the early remodeling mechanism of atrial fibrillation.