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建立了用ＨＰＬＣ柱切换测定血浆中氟康唑的方法。血样用０．２ｍｏｌ·Ｌ－１醋酸溶液简单稀释后注入填充ＬｉＣｈｒｏｍｐｒｅｐＲＰ２（２５～４０μｍ）的预柱上，用蒸馏水冲洗出血浆中蛋白和其它极性成分。切换后，浓缩在预柱上的氟康唑被流动相甲醇－０．２ｍｏｌ·Ｌ－１醋酸按（ｐＨ２．７）（５０：５０）反冲到分析柱ＳｈｉｍｐａｃｋＣＬＣ－ＯＤＳ上进行分析。预柱用净化液进行清除和再生。本法有很好的精密度与回收率，检测限为０．１２μｇ·ｍｌ－１血浆，日间和日内测定相对标准偏差均小于６％。此分析法已成功地用于测定自愿受试者的氟康唑药代动力学及生物利用度。
A method for the determination of fluconazole in plasma by HPLC column was established. Blood samples were briefly diluted with 0.2 mol·L-1 acetic acid solution and injected into a pre-column packed with LiChromprepRP2 (25-40 μm). The plasma proteins and other polar components were washed out with distilled water. After switching, the fluconazole concentrated on the precolumn was backwashed on the analytical column Shimpack CLC-ODS by mobile phase methanol-0.2 mol·L-1 acetic acid (pH 2.7) (50:50). Pre-column with cleaning fluid for cleaning and regeneration. The method has good precision and recovery, with the detection limit of 0.12μg · ml-1 plasma, and the relative standard deviations (RSDs) were all less than 6% during the day and the day. This assay has been successfully used to determine fluconazole pharmacokinetics and bioavailability in volunteer subjects.