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本研究从十多种中药多糖中筛选出防风多糖有效部位JBO-6(以下简称JBO-6),进行了抗肿瘤免疫作用实验研究.结果表明JBO-6体外无抑制肿瘤细胞生长作用,而体内有明显抑制肿瘤生长作用.JBO-6具有体外免疫增强作用即能单独明显刺激小鼠脾淋巴细胞增殖但单独不刺激小鼠胸腺细胞增殖.而与ConA合用时则对小鼠胸腺细胞增殖有协同作用;能刺激裸鼠脾淋巴细胞增殖,此外还能促进小鼠腹腔MΦ吞噬作用及细胞毒作用,促进小鼠NK活性.JBO-6具有体内免疫增强作用,即增加小鼠脾重,能提高小鼠胸腺细胞对ConA的增殖反应;能提高脾淋巴细胞对LPS增殖反应.此外还能提高小鼠外周血L3T4细胞百分率及K3T4/Lyt2比例,小鼠腹腔MΦ吞噬功能及细胞毒作用、NK细胞活性及IL-1、2活性.
In this study, JBO-6 (hereinafter abbreviated as JBO-6), an effective fraction of polysaccharides from wind-proof polysaccharides, was screened from over a dozen Chinese herbal polysaccharides and tested for anti-tumor immunity. The results showed that JBO-6 did not inhibit the growth of tumor cells in vitro, but in vivo It can obviously inhibit the growth of tumor. JBO-6 can enhance the proliferation of mouse spleen lymphocytes alone but does not stimulate the proliferation of mouse thymocytes alone. In combination with ConA, it has a synergistic effect on the proliferation of mouse thymocytes. Role; can stimulate the proliferation of spleen lymphocytes in nude mice, in addition can promote peritoneal MΦ phagocytosis and cytotoxicity in mice, promote mouse NK activity. JBO-6 has in vivo immune enhancement, that increase the spleen weight of mice, can improve Proliferation of ConA in mouse thymocytes; increase of LPS proliferation in splenic lymphocytes. In addition, the percentage of peripheral blood L3T4 cells and K3T4/Lyt2 ratio in mice, the peritoneal MΦ phagocytosis and cytotoxicity, and NK cells in mice Activity and IL-1, 2 activity.