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选用两个生产上主栽马铃薯品种“鲁引 1号”和“鲁引 4号”的试管微型薯为材料 ,通过农杆菌介导成功地将菜豆几丁质酶基因导入到马铃薯中。试管微型薯薯片与农杆菌共培养 3d后 ,转到含卡那霉素 10 0mg/L的分化培养基上诱导不定芽分化。待抗性芽长到 1 0～ 1 5cm高时 ,转入含卡那霉素 10 0mg/L的液体培养基中进行生根筛选。经过分化和生根两轮筛选的转化植株的PCR检测结果均为阳性 ,而未经转化的对照植株为阴性 ,证明该筛选系统是可靠的。早熟品种鲁引 1号的转化频率明显高于晚熟品种克新 4号 ,其最高转化频率为 4 1 0 % ,平均每个外植体可得到 3 85株转化苗。转化植株的抗病性鉴定正在进行中
Two mini-tubers of cucumber variety “Luyin 1” and “Luyin 4” were used as materials. Agrobacterium tumefaciens was used to successfully introduce the bean chitinase gene into potato. After in vitro culture for three days, the microtuber potato chips were inoculated with Agrobacterium and transferred to the differentiation medium containing 100 mg / L kanamycin to induce adventitious bud differentiation. To be resistant to buds grow to 10 ~ 15cm high, transferred to kanamycin 10mg / L liquid medium for rooting screening. The PCR results of the two transformed plants after differentiation and rooting were all positive, while the untransformed control plants were negative, which proves that the screening system is reliable. The transformation frequency of the early maturing cultivar Lu Yin 1 was significantly higher than that of the late-maturing cultivar Kexin 4, with the highest frequency of transformation being 41.0%, with an average of 855 transformed plants per explant. Resistance of transformed plants is under identification