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大豆胞囊线虫病的鉴定受条件和方法的制约,一直是大豆抗病资源筛选和抗病品种选育的限制因素之一,与抗病基因紧密连锁的分子标记的发现,为抗病鉴定提供了高效而准确的鉴定方法。以抗大豆胞囊线虫病不同世代的育种分离群体为材料,采用人工接种鉴定和抗病基因型鉴定相结合的方法,探讨了标记基因型在不同分离世代的鉴定效率。结果表明:随着分离群体世代的增加,抗病个体在分离群体中所占比例呈上升趋势,分离世代的卡方测验表明抗病性由2个隐性基因控制;基于抗病基因Rhg1开发的Indel标记rhg1-I4和SCN_Res Bridge对表型的检测效率达到88.89%,显著高于Satt309(50.00%);随着育种群体世代的增加,标记检测效率有所提高。不同标记组合并没有提高标记的检测效率。结果证明基于抗病基因序列开发的功能性标记在大豆抗胞囊线虫病育种中有广泛的应用前景。
Identification of soybean cyst nematode disease is restricted by the conditions and methods, which has been one of the limiting factors in screening of disease-resistant soybean and breeding of resistant varieties. The discovery of molecular markers closely linked with disease-resistance genes provided the basis for disease resistance identification Efficient and accurate identification method. The segregation population of different generations of soybean cyst nematode resistance was used as material. The combination of artificial inoculation and resistance genotype identification was used to investigate the identification efficiency of marker genotypes in different segregation generations. The results showed that with the increase of segregating population generations, the proportion of disease-resistant individuals in the segregating population showed an upward trend. The chi-square test of segregating generations showed that disease resistance was controlled by two recessive genes. Based on the resistance gene Rhg1 The phenotypic efficiency of Indel markers rhg1-I4 and SCN_Res Bridge was 88.89%, which was significantly higher than that of Satt309 (50.00%). With the increase of breeding population, the efficiency of marker detection increased. Different marker combinations did not improve the detection efficiency of the marker. The results demonstrated that the functional markers developed based on the sequences of resistance genes have broad application prospects in soybean cyst nematode breeding.