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目的:探讨胃旁路术对非肥胖2型糖尿病GK大鼠肝脏葡萄糖激酶(glucokinase,GCK)表达的影响。方法 :雄性GK和Wistar大鼠各20只,随机分为GK手术组(GO组)、GK假手术组(GS组)、Wistar手术组(WO组)和Wistar假手术组(WS组),每组10只。检测术前及术后第1、4、8周各组空腹血糖和血清胰岛素水平,术后8周取大鼠肝脏组织,采用RT-PCR检测肝脏GCK mRNA表达水平。结果:GO组术后第1周空腹血糖已开始下降,术后第1、4、8周分别由术前(8.73±1.30)mmol/L下降到(6.84±0.89)、(6.27±0.93)、(5.86±0.57)mmol/L(P<0.05);随访过程中,GO组空腹血清胰岛素较术前和相应时间点GS组均有所升高,尚未达统计学差异(P>0.05);GO组术后第8周肝脏GCK mRNA表达水平明显高于GS组(P<0.05)。结论:胃旁路术能显著改善非肥胖2型糖尿病大鼠的糖代谢,手术的降糖作用可能与肝脏葡萄糖激酶表达增加有关。
Objective: To investigate the effect of gastric bypass on the expression of hepatic glucokinase (GCK) in non-obese type 2 diabetic GK rats. Methods: Twenty male and female GK and Wistar rats were randomly divided into GK group (G0 group), GK group (G0 group), Wistar group (W0 group) and Wistar group Group of 10. Fasting blood glucose and serum insulin levels were measured before and 1, 4 and 8 weeks after operation. Liver tissues were obtained at 8 weeks after operation. The expression of GCK mRNA in liver was detected by RT-PCR. Results: The fasting blood glucose level of GO group began to decrease in the first week after operation, and decreased from (8.73 ± 1.30) mmol / L to (6.84 ± 0.89) and (6.27 ± 0.93) (5.86 ± 0.57) mmol / L (P <0.05). During the follow-up, fasting serum insulin levels in GO group were higher than those in GS group before and at the corresponding time point, and the difference was not statistically significant (P> 0.05) The expression level of GCK mRNA in liver in the 8th week after operation was significantly higher than that in the GS group (P <0.05). Conclusion: Gastric bypass surgery can significantly improve glucose metabolism in non-obese type 2 diabetic rats. The hypoglycemic effect may be related to the increase of hepatic glucokinase expression.