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目的探讨戊乙奎醚对感染性脑损伤大鼠大脑皮质细胞凋亡的影响。方法60只SD雄性大鼠随机分为5组:假手术组,模型组,戊乙奎醚低、中、高剂量组,每组12只。颈内动脉注射脂多糖150μg(0.15 mL)建立大鼠感染性脑损伤模型。戊乙奎醚低、中、高剂量组在注射脂多糖后即刻和12h分别经腹腔注射戊乙奎醚0.05、0.15和0.45 mg·kg~(-1),假手术组和模型组给予等容量生理盐水。注射脂多糖24h后处死动物,测定脑组织含水率、皮质细胞凋亡指数、Bcl-2和Bax的表达水平、iNOS mRNA的水平。结果与假手术组相比,模型组大鼠脑组织含水率、凋亡指数、Bax及iNOS mRNA表达水平均增加,Bcl-2表达减少(P<0.05)。与模型组相比,戊乙奎醚低剂量组上述指标组间差异不明显(P>0.05),中剂量组和高剂量组脑组织含水率、凋亡指数、Bax及iNOS mRNA表达水平均减少,Bcl-2表达增加,差异有显著意义(P<0.05)。结论戊乙奎醚0.15和0.45mg·kg~(-1)可抑制感染性脑损伤大鼠大脑皮质细胞凋亡。
Objective To investigate the effect of penehyclidine on cerebral cortical neuronal apoptosis in rats with infectious brain injury. Methods Sixty SD male rats were randomly divided into 5 groups: sham operation group, model group, penehyclidine low, medium and high dose group, 12 rats in each group. The rat model of septic brain injury was established by injecting lipopolysaccharide 150μg (0.15 mL) into the internal carotid artery. Penehyclidine low, medium and high dose groups were injected intraperitoneally with penehyclidine 0.05, 0.15 and 0.45 mg · kg -1 immediately after injection of lipopolysaccharide and 12 h respectively. Sham-operation group and model group were given equal volume Physiological saline. Animals were sacrificed 24h after injection of lipopolysaccharide, and the water content of brain tissue, apoptosis index of cortical cells, the expression of Bcl-2 and Bax and the level of iNOS mRNA were measured. Results Compared with the sham operation group, the water content, apoptosis index, the expression of Bax and iNOS mRNA in the model group increased and the expression of Bcl-2 decreased (P <0.05). Compared with the model group, there was no significant difference in the above indicators between the low dose penehyclidine group (P> 0.05), the water content, apoptosis index, Bax and iNOS mRNA expression in the medium dose group and high dose group were decreased , The expression of Bcl-2 increased, the difference was significant (P <0.05). Conclusion Penehyclidine 0.15 and 0.45 mg · kg -1 can inhibit the apoptosis of cerebral cortex in rats with brain injury induced by infectious.