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丝虫幼虫置液氮内冷冻保存国外已有报道,国内报道甚少。1985年我们参考国外有关文献,并改进一些方法,进行了周期型马来丝虫微丝蚴液氮内冷冻保存的试验,取得初步结果,现报告如下。材料和方法一.微丝蚴(mf)的收集及冷冻保护液用腹腔内灌洗法从马来丝虫感染的长爪沙鼠腹腔液中收集微丝蚴。实验前用灭菌的37℃Earle 氏液冲洗微丝蚴,并于1500rpm 离心10分钟,弃去上清液,反复三次。
Microfilaria larvae liquid cryogenic preservation has been reported abroad, few reports. In 1985 we refer to foreign literature, and to improve some of the methods were carried out Cyclophilus malaria microfilariae liquid nitrogen cryopreservation experiment, and achieved initial results are as follows. MATERIALS AND METHODS A. Collection and cryoprotectant solution of microfilariae (mf) Microfilaria were collected from peritoneal cavity of Malayan worm infected Meriones unguiculatus by peritoneal lavage. Prior to the experiment, microfilariae were washed with sterile Earle’s solution at 37 ° C and centrifuged at 1500 rpm for 10 minutes. The supernatant was discarded and repeated three times.