对35例急性淋巴细胞白血病(ALL)患儿血液标本进行了DNA含量流式细胞术(FCM)分析。依据DNA指数(DI)分类,35例中亚二倍体5例,二倍体22例,超二倍体8例。其中亚二倍体组患儿初诊时肝脾肿大及白细胞计数显著高于其它二组。患儿完全缓解(CR)后其DI值恢复正常。依据DNA含量做细胞周期分析表明,获CR后患儿的增殖期细胞百分数(P％)较初治时有显著下降。流式细胞术标本制做简单,便于临床常规使用。 35 cases of children with acute lymphoblastic leukemia (ALL) blood samples were analyzed by flow cytometry (FCM). According to the DNA index (DI) classification, 35 cases of diploid in Central Asia in 5 cases, diploid in 22 cases, 8 cases of diploid. The sub-diploid group of children with hepatosplenomegaly and white blood cell count was significantly higher than the other two groups. Children with complete remission (CR) DI value returned to normal. Cell cycle analysis based on DNA content showed that the percentage of cells in proliferating phase (P%) after CR was significantly lower than that at initial treatment. Flow cytometry specimens made simple, easy to use clinical routine.