测定核黄素含量的方法有生物法、微生物法、极谱法、光黄素法和荧光法等。但这些方法大都只适用于测定制剂中的核黄素,对于发酵液中核黄素的测定,其中仅有萤光法尚较适宜,国内核黄素研究部门多用此法。本文目的是研究用普通的光电比色计来进行发酵液中核黄素含量的快速测定。考虑到核黄素本身的氧化还原性,即通过 Methods for the determination of riboflavin content include biological methods, microbial methods, polarography, photofluorescence and fluorescence methods. However, most of these methods are only suitable for the determination of riboflavin in the preparation. For the determination of riboflavin in the fermentation broth, only the fluorescent method is more suitable, and the domestic riboflavin research department uses this method more. The purpose of this paper is to study the rapid determination of riboflavin content in broth using a common photoelectric colorimeter. Considering the redox properties of riboflavin itself, ie