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作者以质粒pUC19为载体,从重组cos质粒pCM1015中分离编码人巨细胞病毒(HCMV)150kd磷蛋白(pp150)主要DNA片段—EcoRI—Y片段,构建了该片段的克隆,依据插入方向不同分别为重组质粒pHCY1和pHCY2,经限制性内切酶酶切分析及Southern印迹杂交鉴定,克隆构建成功,插入片段大小无改变。采用微机系统分析pp150抗原决定簇DNA编码区段的限制性酶切位点,表明该编码区段具有76种限制性内切酶位点,酶切片段大小在4bp～2667bp之间。
The plasmid pUC19 was used as a vector to isolate the major DNA fragment encoding the 150kd phosphoprotein (pp150) of human cytomegalovirus (HCMV) -EcoRI-Y from the recombinant cosmid pCM1015. The cloned fragment was constructed according to the different insertion directions The recombinant plasmids pHCY1 and pHCY2 were identified by restriction endonuclease digestion and Southern blot hybridization. The clones were successfully constructed and the size of the inserted fragment was unchanged. The restriction enzyme sites of pp150 antigenic determinant DNA coding region were analyzed by computer system, which indicated that the coding region had 76 restriction enzyme sites and the size of the digested fragment was between 4 bp and 2667 bp.