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以厚皮甜瓜“伽师”为试材,研究了6-苄氨基嘌呤(6-BA)、硝酸银(AgNO3)对诱导不定芽的影响,建立以子叶为外植体的快速高效再生体系。选取5d的“伽师”无菌苗子叶,在添加了不同浓度6-BA的MSB培养基中诱导不定芽;并且探讨了在最适6-BA浓度的培养基中添加不同浓度AgNO3对诱导不定芽的影响。结果表明:甜瓜子叶外植体接种在MSB+1.5mg·L~(-1) 6-BA诱导分化培养基上,诱导率可达75%;添加不同浓度AgNO3诱导不定芽,以MSB+1.5mg·L~(-1)6-BA+2.0mg·L~(-1) AgNO3最适,诱导率可达65%,产生单芽较多,缩短了再生周期。丛生芽或单生不定芽转至MSB+0.1mg·L~(-1) 6-BA+0.1mg·L~(-1) GA3的培养基中伸长,不定芽均可伸长且生长状况良好;再将单芽转至MSB+0.01mg·L~(-1) NAA培养基进行生根。
The effects of 6-benzylaminopurine (6-BA) and silver nitrate (AgNO3) on the induction of adventitious buds were studied by using the muskmelon “Jiashi” as test material, and the rapid and efficient regeneration with cotyledons as explants was established system. Adventitious buds were induced in MSB medium supplemented with 6-BA with different concentrations of 5-day-old “Jiashi” sterile seedling leaves, and the effects of adding different concentrations of AgNO3 to the optimal 6-BA medium Induction of adventitious buds. The results showed that explants of melon cotyledons were inoculated on MSB + 1.5mg · L -1 6-BA inducing differentiation medium with the induction rate up to 75%. Adventitious buds were induced with different concentrations of AgNO 3, · L 6 -1 BA + 2.0 mg · L -1 AgNO 3, the induction rate was up to 65%, more single buds were produced and the regeneration cycle was shortened. The shoots or adventitious buds were transferred to MSB + 0.1 mg · L -1 (-1) 6-BA + 0.1 mg · L -1 GA 3 medium for elongation, and adventitious buds could elongate and grow Good; then single shoots were transferred to MSA + 0.01mg · L -1 NAA medium for rooting.