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以细枝木麻黄3个无性系水培苗作为材料,研究了硫酸卡那霉素(kanamycin sulfate,Km)对细枝木麻黄愈伤组织诱导、增殖和芽分化的影响,以及头孢霉素(cefotaxime,Cef)、羧苄青霉素(carbenicillin,Cab)和氨苄青霉素(ampicillin,Amp)对农杆菌的抑制效果。结果表明,Km对细枝木麻黄愈伤组织诱导适宜的选择压力是20mg/L,Km选择性筛选愈伤组织增殖的适宜质量浓度为40mg/L,愈伤组织芽分化的选择压力是40～50mg/L。在抗生素抑菌效果方面,Cef质量浓度为200mg/L、Cab300mg/L和Amp500mg/L能完全抑制农杆菌的生长。综合比较,对细枝木麻黄遗传转化研究最理想的抑菌剂是Cef,使用质量浓度低且能有效抑制根癌农杆菌的生长。
The effects of kanamycin sulfate (Km) on callus induction, proliferation and bud differentiation of Ephedra sinica were studied by using three clones of Tricholoma matsutake Cefotaxime, Cef), carbenicillin (Cab) and ampicillin (Amp) on Agrobacterium. The results showed that the suitable selection pressure of Km for callus induction of twig was 15mg / L, Km was 40mg / L for selective screening of callus, the selection pressure of callus differentiation was 40 ~ 50mg / L. In antibacterial antibacterial effect, Cef concentration of 200mg / L, Cab300mg / L and Amp500mg / L can completely inhibit the growth of Agrobacterium. In conclusion, Cef was the most effective bacteriostatic agent for the genetic transformation of the twigs Tricholoma matsutake, with low concentration and effective inhibition of Agrobacterium tumefaciens growth.