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目的 :研究三七总皂甙 (PNS)对减轻颅脑创伤后脑组织损伤机制。方法 :改良八木国夫法检测家兔脑损伤后 2 4h脑组织中的丙二醛 (MDA)含量、脑组织含水量及脑组织超微结构改变 ,观察PNS对上述指标的影响。结果 :脑损伤后 2 4h脑损伤 +PNS组及对照组脑组织中MDA含量分别为 (81.43± 9.72 )nmol/g湿脑 ,(76 87± 8.31)nmol/g湿脑 ,明显低于脑损伤组MDA含量 (10 6 .5 2± 17.6 8)nmol/g湿脑 ,(P <0 .0 1) ;脑损伤 +PNS组及对照组脑组织中水含量分别为 78.97%± 0 .91% ,78.35 %± 0 .89% ,明显低于脑损伤组 80 .74± 0 .98(P <0 0 1)。损伤脑组织中MDA含量与伤后脑水肿程度呈正相关 (P <0 .0 1) ,同时脑损伤组出现明显的脑组织超微病理改变。结论 :PNS能够降低颅脑损伤后自由基的产生 ,减轻脑水肿和脑组织病理损害 ,对颅脑损伤具有一定的脑保护作用。
Objective: To study the mechanism of panax notoginseng saponins (PNS) in reducing brain injury after traumatic brain injury. Methods :Improved Yagi Koufu method was used to detect malondialdehyde (MDA) content in brain tissue, water content in brain tissue and brain ultrastructure changes 24 hours after brain injury in rabbits. The effects of PNS on these parameters were observed. Results: The content of MDA in the brain tissue of brain injury + PNS group and control group was (81.43 ± 9.72) nmol/g wet brain and (76 87 ± 8.31) nmol/g wet brain, which was significantly lower than that of brain damage. The MDA content in the group was (106.52±16.88%) nmol/g wet brain (P<0.01); the brain water content in brain injury+PNS group and control group was 78.97%±0.91%, respectively. 78.35 % ± 0.89 %, significantly lower than 80.74 ± 0 .98 in brain injury group (P <0 01). The content of MDA in the damaged brain tissue was positively correlated with the degree of brain edema after injury (P < 0.01). At the same time, the pathological change of the brain tissue was obvious in the brain injury group. Conclusion : PNS can reduce the free radical production after brain injury, relieve cerebral edema and pathological damage of brain tissue, and has a certain brain protection effect on brain injury.